What is a safety hazard associated with this lab session. Thirtythree laboratories collaborated in the interlaboratory proficiency tests for characterization of iodine value, while thirtytwo laboratories for characterization of slip melting point. Determination of melting point determining the melting point of a compound is one way to test if the substance is pure. The melting point of a substance is the temperature at which the material changes from a solid to a liquid state. Nov 19, 2008 a simple guide to help you determing the melting point of solid products accurately.
Determination of melting point of fat determination of saponification value determination of unsaponifiable matter determination of acid value. The sample is a clear liquid and no solid particles remain visible clear point. Collaborative trials on a standard testing procedure, aocs cc325, revealed the inadequacy for palm oil of the temperature range of 410 c specified in the procedure and its fractions. Aacc approved methods of analysis, 11th edition aacc method.
Animal and vegetable fats and oils determination of. The most important factor appears to be the difference in tempering temperatures. In all cases, data points are randomly distributed about. The determination of slip melting point smp in refined, bleached, and deodorized palm oil is a common and regular requirement in the. Collaborative trials on a standard testing procedure, aocs cc3. Determination of stability of blends with high percentage of. The melting temperature of palm stearin is usually determined by the slip melting point smp.
Palmbased standard reference materials for iodine value and. Determination of slip melting point in palm oil blends by partial least. It was designed to fit the needs of the accurate characterization of. This method is applicable to palm oil and hard fats, including palm. Slip melting point determination of lipstick measuring the slip melting point smp is important to the quality control in the cosmetic and food industries.
Determination of melting point of vegetable oils and fats. This method determines the slip point of a sample and is applicable to such fats as coconut oil. In this study the relationship between slip melting point smp and fatty acid composition of blends before and afrer interesterijication were investigated. A secondary effect on the slip melting point was the height of fat in the capillary tube. Melting point determination with animation duration. Pdf slip melting point estimation of fat blends before. A variety of methods exist for the determination of the melting point mp of fats.
Preparing a sample of melting point determination melting point samples are prepared in a capillary tube that is closed on one end a melting point capillary. When determining the melting point of a substance it is necessary to select one stage in the melting process which will be used to define the melting point. In general, impurities lower and broaden the melting range. Automatic measurements of of up to six samples simultaneously and observe your measurements via video, or store and replay it for measurement verification. Before use, clean the tubes thoroughly by washing them successively with a mixture of chromic acid, water and acetone, and then dry them in an oven. The mp55 and mp80 act as an automatic instrument for slip melting point determination according to the following measurement principle. Place a small quantity 116 inch in tube of the solid to be melted in a capillary tube labeled melting point tubes. The melting point or, rarely, liquefaction point of a substance is the temperature at which it changes state from solid to liquid.
A catalog is available as a download from their website. Objective the slip point open tube melting point is an index of temperature at which fat softens or becomes sufficiently fluid to slip or run. The melting point m565 for the automatic determination of melting and boiling points is designed for true, professional quality control. No linear relationship existed between the calculated and experimental results of the blends before interesterification, but smp of the blends after interesterification could be estimated with high r0. Slip melting point the slip melting points of nie, eie and cie stearin fraction is given in table 1. Impurities lower the melting temperature of a substance, and increase the melting point range. Scope this method is used in the characterization of palm oil in international trade. Pdf slip melting point estimation of fat blends before and. Repeatability and reproducibility relative standard deviations were found to be good and acceptable, with. A pure substance generally has a melting range the difference between the temperature where the sample starts to melt and the temperature where melting is. This tube needs to be sealed at one end presealed tubes should be available in the laboratory, or, an open capillary can be sealed by inserting. Effects of using different methods of determination are also shown.
In this study, the cie stearin fraction has the highest iv and the lowest slip melting point as shown in table 1. Sep 22, 2008 this work described study protocols on the production of palmbased standard reference materials for iodine value and slip melting point. Parameters of crude palm oil using nearinfrared spectroscopy. Slip melting point determination of palm stearin buchi labortechnik. Melting point, boiling point, cloud point, and slip melting point measuerement on the melting point excellence instruments. Determination of melting point of vegetable oils and fats by. Pdf in this study the relationship between slip melting point smp and fatty acid composition of blends before and after interesterification. Palm stearin is a mixture of fats, fractionated from palm oil.
The property values for each rm are characterised through interlaboratory comparison programmes involving nationwide and worldwide laboratories based on mpob test methods. Materials meltemp melting point apparatus capillary tube unknown sample table of melting point data laboratory notebook theory the melting point mp of a substance is one of the physical properties that chemists use to identify a substance. Our compliant melting point instrument offers an automatic determination of melting and boiling points for professional quality control. The melting characteristic of palm stearin is an important quality parameter and depends on its content of different fats. Meltingpoint determination of fat products springerlink. Slip melting point smp of structured fats, noninteresterified blends, and tallow was determined according to aocs method cc 325. Animal and vegetable fats and oils determination of melting point in open capillary tubes slip point 1 scope this international standard specifies two methods for the determination of the melting point in open capillary tubes, commonly known as the slip point, of animal and vegetable fats and oils referred to as fats hereinafter. Determination of quality parameters of crude palm oil. Identification of triacylglycerol profile, solid fat. Aacc international approved methods table of contents. Animal and vegetable fats and oils determination of melting point in open capillary tubes. To get the best performance from the smp30 please read these instructions carefully before use. Extracted cocoa butter like fat is heated to liquid fraction and filled into capillary tube for 1 cm length triplo. Weigh in a previously dried and tared dish about 5 10g of oil or fat which.
At the melting point the solid and liquid phase exist in equilibrium. Slip melting point meaning slip melting point defin. Crush the solid substance into a fine powder using the end of a test tube and a watch. For a given substance, the melting point of its solid form is the same as thefreezing point of its liquid form, and depends on such factors as the. One will be using instruments that will be heated to high temperatures. Pure compounds were seen to have a narrow melting range, while. Melting point determination purpose identify an unknown compound by its melting point. For a given substance, the melting point of its solid form is the same as thefreezing point of its liquid form, and depends on such factors. The determination of slip melting point smp in refined, bleached, and deodorized palm oil is a common and regular requirement in the manufacture of this oil.
Slip melting point estimation of fat blends before and after interesterification based on their fatty acid compositions article pdf available in journal of food lipids 103. Physical and chemical characteristics of oils, fats, and waxes, champaign, illinois, aocs press, 2006. Mettler toledos excellence melting point systems offer the possibility of measuring these thermal values accurately, automatically, melting point excellence and with just one click of the button. An apparatus is described for the determination of melting and freezing points of small amounts of organic substances. Because the melting point and oxidative stability are related to the degree of unsaturation, iv provides an estimation of these quality factors. Determination of melting points according to pharmacopeia.
Science experiment melting point and freezing point of water duration. To determine the melting point of a solid substance. Before discarding the packaging check that all parts are present and correct. The capillary tubes was cooled to at 4 100c for 16 hours. Determination of slip melting point in palm oil blends by. A meniscus is visible at the sample surface but solid particles remains visible meniscus point. Here, we demonstrate the feasibility to employ the m565 for smp measurements of a lipstick sample. Determination of moisture content determination of specific gravity determination of refractive index determination of flash point 6.
Palmbased standard reference materials for iodine value and slip melting point article pdf available in analytical chemistry insights 33. Description of melting process what happens when a compound melts. Reference materials for iodine value and slip melting point, analytical chemistry insights, 2008, 3, 1273. Factors affecting slip melting point of palm oil products. Melting point apparatus smp30 instructions for use 1. Melting point m565 slip melting point determination of. Jun 27, 2016 to determine the melting point of a solid substance. Melting point capillary method the melting point determined by the capillary method is the temperature at which the last solid particle of a compact column of a substance in a tube passes into the liquid phase. Melting point is the temperature at which a solid substance melts or fuses. Iodine value iv the iodine value is a measure of the relative degree of unsaturation in oil components, as determined by the uptake of halogen. For hydrogenated oils and for some high melting palm stearins, tempering has no effect. In this lab we used melting points to determine the purity of organic compounds. Melting point, boiling point, cloud point, and slip melting. Introduction thank you for purchasing this piece of stuart equipment.
Ir spectroscopy and chemometrics for physical property. So, one should take precautions when handling these instruments. The iodine value of stearin fractions are related to slip melting point of fats. Melting point m565 slip melting point determination of palm. Method for inspection of animal and vegetable oils and fats for export part 5. It is a mixture of different fully natural ingredients 1 and mainly used as an edible, transfat free and thus healthy alternative to partially hydrogenated plant oils 2. The effect of different factors affecting the slip melting point of palm oil has been evaluated. Slip melting point of blends were calculated using these constants and fatty acid compositions and then compared with the experimental values. For the slip melting points, the consensus values and uncertainties were 35.
Melting point of unknown mixed with melting point of unknown. Animal and vegetable fats and oils determination of melting point in open capillary tubes slip point animal and vegetable fats and oils determination of melting point in open capillary tubes slip point skip to main content. Slip melting point 15c max cloud point 4c max free fatty acids 0. Discussion for melting point lab analysis of mixed.
The melting point of a substance depends on pressure and is usually specified at a standard pressure such as 1 atmosphere or 100 kpa. This experiment is essentially an exercise in recrystallization and identification, by melting point and mixed melting point determination, an unknown ketone that has been transformed into either its 2,4dinitrophenylhydrazone or semicarbazone derivative. After reach ing the reaction equilibrium point, to inactivate the catalyst, 2% ww. Factors affecting slip melting point of palm oil products springerlink. This method determines the slip point of a sample and is applicable to such fats as coconut oil, stearin, hydrogenated fats, and hard tallows. The higher the degree of unsaturation, the lower the slip melting point will be. Data obtained from the interlaboratory comparison programmes. For example, the melting points of samples of benzoic acid contaminated with known.
It is determined by casting a 10 mm column of the solid in a glass tube with an internal diameter of about 1 mm and a length of about 80 mm, and then immersing it in a temperaturecontrolled water bath. The influence of different tempering temperatures on slip point values is, however, dependent on the nature of the sample. A second reason to determine a compounds melting point is for a rough measure of purity. Improvement of physical properties of palm stearin and. The slip point is an index of the temperature at which fat softens and becomes sufficiently fluid to slip in an open capillary tube. The determination of melting points melting points will be determined by using one of the digimelt units figure 1. When prescribed in the monograph, the same apparatus and method are used for the determination of other factors, such. Pdf slip melting point estimation of fat blends before and after. A pure substance generally has a melting range the difference between the temperature where the sample starts to melt and the temperature where melting is complete of one or two degrees. For example, the melting points of samples of benzoic acid contaminated with known quantities of acetanilide are summarized in table 6. Reproducibilities of the mettler dropping point and softening point were considered excellent and for slip point, poor. It is used to characterize cosmetics, fats, and other substances with a waxy consistency. Aacc approved methods of analysis, 11th edition aacc. The general method is to the heat the sample indirectly by placing the prepared sample either packed in a glass capillary or on a glass cover slip in or on a.
This work described study protocols on the production of palmbased standard reference materials for iodine value and slip melting point. These include the wiley mp aocs method cc 238, open capillary slip point, softening point and mettler dropping point. Slip melting point determination of palm stearin palm stearin, the solid fraction of palm oil, is a natural source of hard fat. Physicochemical properties of palm oil and palm kernel oil. Palmbased standard reference materials for iodine value. Slip melting point determination aocs cc 3 25, 2003 slip melting point smp was determined according to aocs official methods cc 3 25, 2005. Cloud point the slip melting point is the melting point of waxy solids and is defined as the temperature at which a sample has softened sufficiently to rise in an open capillary tube.
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